منتجات

كاشف الأجسام المضادة CD56 للكيمياء المناعية

جسم IHC - CD56

مراقبة إيجابية:
Appendix
التعريب الخلوي:
Cytoplasm/cell membrane
طلب:
IHC-P
الجسم المضاد الثانوي:
iVision™
رقم الكتالوج:
AM0056
الأنواع المضيفة:
Mouse
استنساخ:
56C04
المواصفات / مل:
1、3、6、0.2（Concentrated）

الاستفسار الآن

تفاصيل المنتج

【Product name】

IHC Antibody --CD56

【Packing specification】

Code	Clone	Specifications
AM0056	56C04	0.1ml, 0.2ml, 1ml, 1.5ml, 3ml, 6ml, 11ml, 30ml
AM0211	123C3	0.1ml, 0.2ml, 1ml, 1.5ml, 3ml, 6ml, 11ml, 30ml
AR0466	SD361	0.1ml, 0.2ml, 1ml, 1.5ml, 3ml, 6ml, 11ml, 30ml

【Intended use】

For Research use only. CD56 Antibody reagent is intended for use to qualitatively identify CD56 by microscopy in sections of FFPE tissue using immunohistochemical detection system.

【Principle】

CD56 is a single transmembrane glycoprotein also known as N-CAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. In normal tissue, anti-CD56 labels neurons, glia, schwann cells, NK (natural killer) cells, and a subset of T-cells. CD56 expression can be seen in most NK cell neoplasms, certain subtypes of T-cell lymphoma and in some plasma cell neoplasms. Add the primary antibody to bind the antigen on tissue sections, and then use HRP labeled secondary antibody binding primary antibody to form the secondary antibody-primary antibody-antigen complex. When DAB chromogenic solution is added, HRP reacts with enzyme substrate to produce brown insoluble reaction product, which indirectly indicating the existence of antigen.

【Main components】

Immunoglobulin, antibody diluent

【Storage】

Store at 2～8℃ for 18 months。

【Sample requirements】

FFPE tissues are usually cut into sections as thin as 3～5μm with a microtome. These sections are then mounted onto glass slides that are coated with a tissue adhesive.

【Protocol】

1. Sample preparation：Deparaffinize the slides in xylene Ⅰ, Ⅱ, Ⅲ for 5 minutes；Transfer the slides once through 100%, 100%, 95%, 75% alcohols for 2 minutes respectively. Rinse slides with deionized water for 30 seconds.

2. Blocking：Block endogenous peroxidase activity by incubating sections in 3% H₂O₂ solution at room temperature for 5 minutes to block endogenous peroxidase activity. Rinse the slides with deionized water for 30 seconds.

3. Antigen retrieval：Heat the EDTA Antigen retrieval buffer to 100℃. Then place the slides in the boiled buffer and continue to heat for 15～20 min. Naturally cool down for 30 minutes. Rinse the sample with wash buffer.

4. Primary antibody incubation: Drain the slides. Add primary antibody to tissue, incubate at room temperature for 30 minutes. (use antibody diluent or PBS as control). Wash the slides in PBST for 2 times, 5 minutes for each time. If the Primary antibody is concentrated, please dilute it to RTU(ready to use) according to the information on packing.

5. Secondary antibody: Drain the slides. Add secondary antibody to tissue and incubate at room temperature for 20 minutes. Wash the slides in PBST for 2 times, 5 minutes for each time.

6. DAB：Drain the slides. Add DAB to the tissue and incubate at room temperature for 5 min. Rinse slides with deionized water.

7. Hematoxylin staining：Drain the slides. Add Hematoxylin to the tissue and incubate at room temperature for 5 minutes. Rinse slides with water. Use the acid solution for differentiation. Rinse slides with water.

8. جفف الشرائح في 75٪ ، 95 ٪ ، 100 ٪ كحول لمدة دقيقتين . جفف الشرائح. تغطية الأنسجة الملطخة بغطاء باستخدام وسط تصاعد.